Mentors: Munson, Dziubla
Drug delivery of proteins from controlled-release matrices is complicated by the poor stability of the proteins in the solvents used to prepare the matrices, as well as the need to store the protein without degradation.1 One approach has been to use crystalline proteins that do not dissolve in the solvents and can be incorporated into the matrix.2 However, this limits the number of proteins that can be used. In Theme 1, the students will prepare particles of prestabilized protein/sugar matrices that can then be incorporated into biodegradable polymeric matrices. In Theme 2, the release kinetics of the protein, including in vivo stability of the protein upon release, will be studied. In Theme 3, the students will manufacture implants that can serve as drug delivery implants. An essential element of this project is the use of solid-state NMR spectroscopy to evaluate the protein in situ within the matrices, which can then be used to predict protein stability.
(1) Wu, F.; Jin, T. “Polymer-Based Sustained-Release Dosage Forms for Protein Drugs, Challenges, and Recent Advances”, AAPS Pharmscitech 2008, 9, 1218.
(2) Pechenov, S.; Shenoy, B.; Yang, M. X.; Basu, S. K.; Margolin, A. L. “Injectable controlled release formulations incorporating protein crystals”, J. Control. Release 2004, 96, 149.